Anion exchange chromatography columns, HiTrap™ DEAE FFSupplier: Cytiva
17-5055-01EA 133 EUR17-5055-01 17-5154-01
Anion exchange chromatography columns, HiTrap™ DEAE FF
The HiTrap™ DEAE FF column is pre-packed with DEAE Sepharose™ Fast Flow, and is a weak anion exchanger for small scale protein purifications, as well as screening of binding and elutions conditions.
- Fast, easy ion exchange separations either alone or connected in series
- High flow rates and good scale-up potential
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient
- Predictable scale-up
DEAE Sepharose™ Fast Flow is based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.
The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.