Substrate for Western blotting, ECF™Supplier: Cytiva
RPN5785EA 204.97 EURRPN5785
Substrate for Western blotting, ECF™
Electrophoresis Reagents Blotting Reagents
ECF™ substrate is a fluorescent substrate for alkaline phosphatase-based detection of protein blots, also for use with fluorescence scanning equipment. Alkaline phosphatase dephosphorylates ECF substrate to produce a fluorescent product, which localises at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
- Can be used in Western blotting, dot and slot blotting applications
- Comparable sensitivity with chemiluminescence
- Wider dynamic range of fluorescence scanning systems compared with film allows visualisation and quantitation of weak and dark bands in the same scan
- No need of darkroom and film
Delivery information: Provided with reagents sufficient for detecting 2500 cm² membrane: ECF substrate (60 ml) and dilution buffer.