Multimode columns, HiTrap™ Capto™ MMCSupplier: Cytiva
11-0032-75EA 537 EUR11-0032-75 11-0032-73 29-4004-65
Multimode columns, HiTrap™ Capto™ MMC
HiTrap™ Capto™ MMC columns are prepacked with Capto™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
- High dynamic binding capacity at high conductivity
- Selectivity allows binding in the presence of salt
- High volume throughput
- Used to bind proteins at the conductivity of the feed material and to solve specific purification challenges
The medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap™ Capto™ MMC has been optimised for loading sonicated unclarified cell lysate directly on the column.
The Capto™ MMC ligand may interact with proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.