Affinity chromatography column, HiTrap™ IgY purification HP

Supplier: Cytiva
HiTrap™
17-5111-01EA 628.62 EUR
17-5111-01
Affinity chromatography column, HiTrap™ IgY purification HP
Chromatography Columns
HiTrap IgY Purification HP is a 5 ml column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series
  • Packed with a thiophilic adsorption medium, the ligand, 2-mercaptopyridine is coupled to Sepharose High Performance
  • Recovery and purity of IgY are excellent
  • Can be operated with a liquid chromatography system such as ÄKTA design
  • The top and bottom frits are manufactured from porous polyethylene

HiTrap IgY Purification HP is packed with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Delivery information: Connectors for usage with different chromatography systems and other equipment are included.
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