Affinity chromatography columns, rProtein A Protein G GraviTrap™Supplier: Cytiva
28-9852-56EA 759 EUR28-9852-56
Affinity chromatography columns, rProtein A Protein G GraviTrap™
rProtein A protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids.
- High purity and recovery with purity >95% in one step and recovery typically 70 to 80%
- High binding capacity such as protein A/protein G GraviTrap™ ∼35 mg human IgG/column
- Purification of antibodies in 30 min allows to quickly obtain and store the target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs
It includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose™ Fast Flow and 50% Protein G Sepharose™ 4 Fast Flow. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand used. The columns are reusable up to five times.