Matrix metalloproteinase ELISA and activity assays, Biotrak™

Supplier: Cytiva
RPN2631EA 1335 EUR
RPN2631 RPN2610
Matrix metalloproteinase ELISA and activity assays, Biotrak™
Assays ELISAs
Many normal physiological and pathological processes involve breakdown and remodeling of the extracellular matrix. This degradation is due to the matrix metalloproteinase (MMP) family of enzymes. Biotrak™ ELISAs measure MMP protein levels in human serum, plasma, and cell culture samples. Biotrak™ MMP activity assays measures the amount of active MMP enzyme present in complex biological samples in a convenient, plate-based method that is both sensitive and specific.

  • Both ELISA and activity assays are available
  • Convenient, plate-based colorimetric format

MMP-1, human, Biotrak™ assay
• Specific for total MMP-1: Recognises proMMP-1, active MMP-1, and MMP-1/TIMP-1 complex
• Range: 6,25 to 100 ng/ml
• Sensitivity: 1,7 ng/ml
• Suitable for use with serum, plasma, and cell culture supernatant samples
• 5,5 h protocol
MMP-2, human, Biotrak™ assay
• Specific for proMMP-2: Recognises free pro-MMP-2 and MMP-2 complexed to TIMP-2, but not the active form of MMP-2
• No cross-reactivity with MMP-1, -3, -7, -8, -9 and MT1-MMP
• Range: 1,5 to 24 ng/ml
• Sensitivity: 0,37 ng/ml
• Suitable for use with serum, plasma, tissue, and cell culture supernatant samples
• ~3,5 h protocol
TIMP-1, human, Biotrak™ sssay
• Specific for total TIMP-1 free TIMP-1 and TIMP-1 complexed to MMPs
• Range: 3,13 to50 ng/ml
• Sensitivity: 1,25 ng/ml
• Suitable for use with cell culture supernatant, serum, and plasma samples
• 4,5 h protocol
TIMP-2, human, Biotrak™ assay
• Specific for free TIMP-2 and TIMP-2 complexed with active forms of MMPs, but not TIMP-2 complexed with pro-MMP-2. Does not recognize TIMP-1 or TIMP-3
• Also recognises mouse, rat, rabbit, guinea pig, and bovine TIMP-2
• Range: 8 to 128 ng/ml
• Sensitivity: 3,0 ng/ml
• Suitable for use with cell culture supernatant, serum, plasma, and tissue samples
• ~3 h protocol
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