Anti-beta NGF Rabbit Polyclonal Antibody

Supplier: Biosensis
BSENR-093-500EA 302 EUR
Anti-beta NGF Rabbit Polyclonal Antibody
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.

A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Application Information:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF. Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF. The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: beta NGF
Clonality: Polyclonal
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Rabbit
Isotype: IgG
Reactivity: Mouse, Rat, Human, Avian
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