Anti-Mouse AGRP Guinea Pig Polyclonal Antibody

Supplier: Biosensis
BSENGP-029-50EA 312 EUR
Anti-Mouse AGRP Guinea Pig Polyclonal Antibody
AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.

Specificity was demonstrated by immunohistochemistry.
Application Information:
IHC, frozen, PFA fixed material. Not yet tested on paraffin embedded tissue sections. A concentration of 1:1000 to 1:2000 is recommended for IHC with overnight incubations. Permeabilization suggested is 0.1% triton X-100 in blocking buffer. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/ml of AGRP. In rodents such as rat cell terminal staining is most typically observed without cell body staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Mouse AGRP
Clonality: Polyclonal
Conjugation: Unconjugated
Epitope: carboxy domain of Mouse agouti related protein
Host: Guinea Pig
Isotype: IgG
Reactivity: Human, Rat, Sheep
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