Pure Canavalia ensiformis lectin (Con A) from Jackbean.

• Extinction Coefficient (E1%): 280 nm = 11,4 (0,1 M NaCl)(Lit.)
• Isoelectric Point (pl): 4,5, 4,7, 5,0 to 5,1, and 5,4 to 5,5 (several isoforms)(Lit.)
• Inhibitory Carbohydrate: Methyl-a-D-Mannopyranoside to a-D-Mannose to a-D-Glucose
• Carbohydrate Specificity: a-Mannose, a-Glucose, Branched Mannose
• Soluble in water (10 mg/ ml-slightly hazy, colourless solution)(40 mg/ml in 0,01 M PBS, pH 6,8 containing 0,1 mM Ca²⁺, 0,1 mM Mn²⁺)

Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells.

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5,6 or below. Between pH 5,8 and pH 7,0, Con A exists as a tetramer; above pH 7,0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5,6. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins. Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding. Each subunit of Con-A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity.

Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5,0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25,500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding. Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required.

GHS symbol and symbol: GHS08 and danger
Identity test: Passes
Essentially salt free: Passes
Protein content: Report result