Nitro blue tetrazolium chloride (NBT) for molecular biology

Supplier: MP Biomedicals

Synonyms: 5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride

0219399950 0219399991 0219399983
ICNA0219399950EA 28.2 EUR
ICNA0219399950 ICNA0219399991 ICNA0219399983
Nitro blue tetrazolium chloride (NBT) for molecular biology
Nitro blue tetrazolium chloride (NBT)

p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques. p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

  • Solubility: Slightly (10 mg/mL) soluble in water.

Storage temperature: + 4 °C

Formula: C₄₀H₃₀Cl₂N₁₀O₆
MW: 817.65 g/mol
Melting Pt: 189…192 °C (dec., lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00012159
CAS Number: 298-83-9
EINECS: 206-067-4

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