The T7 RiboMAX™ Express RNAi system is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and non mammalian systems.
- siRNA duplexes for mammalian RNAi
- Long dsRNA for non mammalian RNAi
- Produces milligram amounts of double-stranded RNA in 30 minutes
The T7 RiboMAX™ Express RNAi system can be used to synthesise short interfering RNAs (siRNAs) of 21 bp for use in mammalian systems. siRNAs synthesised in vitro have been demonstrated to be as effective as chemically synthesised siRNAs for inducing RNAi in mammalian cells.
Leveringsinformatie: Kit includes RNase A solution, RiboMAX™ Express T7 2X Buffer, RQ1 RNase-free DNase, T7 Express Enzyme Mix, sodium acetate 3,0 M (pH 5,2), pGEM® Express positive control template and nuclease-free water.