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Capillary columns, traditional phases, SLB-5ms, Supelco®

Capillary columns, traditional phases, SLB-5ms, Supelco®

Supplier: Merck

This column provides boiling point elution order with a slight increase in selectivity, especially for aromatic compounds.

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Tris-EDTA (TE) buffer solution for molecular biology, pH 7,4, BioUltra, Supelco®

Supplier: Merck

Tris-EDTA buffer solution has been used for the dilution of DNA samples.[1] It has been used for the elution of purified vector.

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Solvent desorption tubes, Supelco®

Solvent desorption tubes, Supelco®

Supplier: Merck

These LpDNPH cartridges are suitable for sampling carbonyls in ambient, indoor, and industrial atmospheres. The derivatives are eluted from the cartridge in acetonitrile and are analysed by HPLC.

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Sequencing auxiliary vials

Sequencing auxiliary vials

Supplier: Oxford Nanopore Technologies

Additional sequencing buffer, elution buffer, and loading beads.

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Supelco® Discovery® Cyano HPLC Columns

Supplier: Merck

Discovery® Cyano columns have low hydrophobicity for rapid elution of hydrophobic analytes with exceptional peak shape for basic and acidic analytes.

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Pep-Extract™

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

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HPLC columns, SUPELCOSIL™, LC-NH₂, Supelco®

Supplier: Merck

Silica based SUPELCOSIL™ LC-NH₂ columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased.

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Immobilised Iminobiotin Resin

Immobilised Iminobiotin Resin

Supplier: G-Biosciences

Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

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Capillary columns, traditional phases, SPB-5, Supelco®

Supplier: Merck

These bonded poly(5% diphenyl/95% dimethylsiloxane) low phenyl content columns improve thermal stability of the phase, while still providing essentially a boiling point elution order, and a slight increase in selectivity, especially for aromatic compounds.

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Thiophilic adsorption kit and thiophilic resin

Thiophilic adsorption kit and thiophilic resin

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

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Avantor® ACE® UltraCore Biphenyl, Analytical HPLC/UHPLC Columns

Avantor® ACE® UltraCore Biphenyl, Analytical HPLC/UHPLC Columns

Supplier: Avantor

Avantor® ACE® UltraCore Biphenyl are high performing solid-core (core-shell) particle columns, designed for a range of chromatographic applications and is especially well suited for retention of aromatic compounds that elute early on alkyl phases.

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Coronavirus and SARS-CoV-2 direct RT real-time PCR kits, GSD NovaPrime®

Coronavirus and SARS-CoV-2 direct RT real-time PCR kits, GSD NovaPrime®

Supplier: GOLD STANDARD DIAGNOSTICS

This Real-Time RT-PCR assay is intended for the qualitative detection of SARS-CoV-2 from human nasopharyngeal swabs, eluted directly into deionised water without an additional RNA extraction process.

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Filter funnels, MicroFunnel™

Filter funnels, MicroFunnel™

Supplier: Cytiva (Formerly Pall Lab)

Ideal for concentrating organisms in fluid samples. Flat, smooth surface allows for easy elution of organisms or particulate. Collection of organisms or particulate on one planar surface is ideal prior to electron microscopy (SEM) or light microscope (LM) techniques. Ready to use, disposable design with Ø 47 mm filtration membrane eliminate sample cross contamination.

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Purospher® STAR RP-8 HPLC Columns, Endcapped

Purospher® STAR RP-8 HPLC Columns, Endcapped

Supplier: Merck

Purospher® STAR RP-8 endcapped, like Purospher® STAR RP-18 endcapped, is based on high purity silica and has an almost complete surface coverage. Thus, Purospher® STAR RP-8 provides excellent peak symmetry for acidic, basic and even chelating compounds, highest column efficiency in terms of the number of theoretical plates, and exceptional stability from pH 1,5 to 10,5. In addition, they are ideal for a wide range of applications. As the sorbent is less hydrophobic than Purospher® STAR RP-18 endcapped, analytes will typically elute faster on the C8 phase. Purospher® STAR RP-8 endcapped provides enhanced selectivity for positional isomers and symmetrical peak shapes for strongly basic and less hydrophobic compounds.

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Anti-Fukutin Rabbit Polyclonal Antibody

Supplier: Bioss

Fukutin, a secreted protein, is expressed in various tissues in normal individuals. Fukutin colocalizes with a Golgi marker and a granular cytoplasmic distribution, suggesting that fukutin passes through the Golgi before being packaged into secretory vesicles. Fukutin may be located in the extracellular matrix, where it interacts with and reinforces a large complex encompassing the outside and inside of muscle membranes; alternatively, as a secreted protein, fukutin may cause muscular dystrophy by an unknown mechanism. The fukutin gene is expressed at similar levels in control fetal and adult brain, but is much reduced in Fukuyama congenital muscular dystrophy (FCMD) brains. Fukutin deficiency affects the modification of glycosylation of DAG1 (α-dystroglycan), which then cannot localize or function properly and may be degraded or eluted from the extracellular surface membrane of the muscle fiber. FCMD is the first human disease known to be caused by an ancient retrotransposal integration. The gene which encodes fukutin maps to human chromosome 9q31.

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Accessories for Vac Elut™ 20 manifold

Supplier: VARIAN

Vac Elut™ 20 lid cover

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Anti-FUOM Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))

Supplier: Bioss

Fukutin, a secreted protein, is expressed in various tissues in normal individuals. Fukutin colocalizes with a Golgi marker and a granular cytoplasmic distribution, suggesting that fukutin passes through the Golgi before being packaged into secretory vesicles. Fukutin may be located in the extracellular matrix, where it interacts with and reinforces a large complex encompassing the outside and inside of muscle membranes; alternatively, as a secreted protein, fukutin may cause muscular dystrophy by an unknown mechanism. The fukutin gene is expressed at similar levels in control fetal and adult brain, but is much reduced in Fukuyama congenital muscular dystrophy (FCMD) brains. Fukutin deficiency affects the modification of glycosylation of DAG1 (α-dystroglycan), which then cannot localize or function properly and may be degraded or eluted from the extracellular surface membrane of the muscle fiber. FCMD is the first human disease known to be caused by an ancient retrotransposal integration. The gene which encodes fukutin maps to human chromosome 9q31.

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FTA® Elute Cards, Whatman™

FTA® Elute Cards, Whatman™

Supplier: QIAGEN

FTA® Elute Cards utilise Whatman FTA® technology that simplifies the handling and processing of nucleic acids. DNA is eluted in an easy step, providing DNA in solution ready for amplification.

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Accessories for Vac Elut™ SPS 24 vacuum manifold

Supplier: VARIAN

Luer plugs, For: Vac Elut™ SPS 24 vacuum manifold

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Bond Elut adapters

Supplier: VARIAN

Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge.

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Solid phase extraction cartridges, Bond Elut™ Sodium Sulphate Drying

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

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PPL Tubes, Bond Elut™

Supplier: VARIAN

Bond Elut PPL (Priority PolLutant) is a modified styrene-divinylbenzene polymer that retains polar analytes, including phenols, for excellent absolute recoveries.

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SPE bulk sorbents, Bondesil™

Supplier: VARIAN

Bond Elut Carbon, bulk, 100 g

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96-well plate, Bond Elut™ Certify II

Supplier: VARIAN

Bond Elut Certify II is an anion exchange/mixed-mode SPE product suitable for extraction of acidic compounds from samples.

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HPLC columns, Purospher®

HPLC columns, Purospher®

Supplier: Merck

Purospher® RP-18 is designed for the separation of problematic basic compounds with simple neutral eluents. In addition Purospher® RP-18 allows the separation of hydrophilic compounds with aqueous mobile phase. Purospher® RP-18 is based upon a high purity, metal-free silica. The multi-step chemical modification and deactivation of the surface, and the polar endcapping, enables a peak-tailing-free elution of basic and chelating compounds. Purospher® RP-18 is not suitable for the separation of acidic compounds. The good chemical stability of Purospher® RP-18 enables the use of mobile phase conditions at pH 8 for a long period of time.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

Elution buffer, Omega Bio-Tek

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Solid phase extraction cartridges, Bond Elut™ Mycotoxin

Supplier: VARIAN

Bond Elut™ Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenone analysis. Results are comparable or superior to competing methods, including immunoaffinity columns (IaC) and charcoal/alumina columns. The sorbent is a proprietary silica-based ion exchange material.

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Solid phase extraction cartridges, Bond Elut™ CN-U

Supplier: VARIAN

Bond Elut CN-U is ideally suited for the extraction of polar compounds from a nonpolar matrix, such as hexane or oils. It is suitable for very polar analytes that may be irreversibly retained on SI or Diol (2OH) SPE phases.

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Vacuum manifold, Vac Elut™ SPS 24

Supplier: VARIAN

Vac Elut™ SPS 24 manifold (with 10×75 mm collection rack), For: 10×75 mm test tubes

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Solid phase extraction cartridges, Bond Elut™ PSA

Supplier: VARIAN

Bond Elut™ PSA is an alkylated amine sorbent that contains two different amino functionalities, one secondary and one primary. This gives a slightly higher pKa and ionic capacity compared to Bond Elut™ NH₂. The PSA functional group is a very effective bidentate ligand and is therefore an excellent sorbent for chelation applications. PSA has a significantly higher carbon load than most amino functional sorbents and thus is a better choice for polar compounds, which retain too strongly on Bond Elut™ NH₂.

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